Coding
Zif-vioB

Part:BBa_K323134:Design

Designed by: Tina Lebar, Tjaa Stoicki   Group: iGEM10_Slovenia   (2010-10-20)
Revision as of 16:50, 23 October 2010 by TinaL (Talk | contribs) (→‎Design Notes)

Zif268_vioB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2834
    Illegal AgeI site found at 3030
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Zif268 zinc finger sequence was ligated into an existing BioBrick vector containing the Elowitz RBS site (Part:BBa_B0034). The vioB gene was obtained by PCR from an existing BioBrick of violacein operon (Part BBa_K274002) and ligated as a back insert into the RBS_zinc-finger construct.

Source

a

References