Coding
Blues-vioA

Part:BBa_K323085:Design

Designed by: Tina Lebar, Tjaa Stoicki   Group: iGEM10_Slovenia   (2010-10-20)
Revision as of 15:10, 23 October 2010 by TinaL (Talk | contribs) (→‎Source)

Blues_vioA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1359
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is composited from the Blues zinc finger gene(Part:BBa_K323136) and the vioA gene, which codes for the first enzyme in the violacein biosynthetic pathway. The resulting proteins are connected with a glycine serine linker. Therefore, the product of the DNA sequence is a chimeric VioA enzyme, which binds to a specific DNA sequence.

Source

The vioA sequence was obtained by PCR from an existing part, which was designed by the Cambridge 2009 iGEM team (Part:BBa_K274002). The Blues zinc finger is a synthetic gene, which our team designed and ordored from GeneArt.

References