Composite

Part:BBa_K314100

Designed by: Tomas Huber, Justin Siegel   Group: iGEM10_Washington   (2010-10-08)
Revision as of 04:12, 23 October 2010 by Spch (Talk | contribs)

High constitutive expression cassette

High constitutive protein expression insert includes f1 origin K314110, a high expression promoter J23100, and the Elowitz standard RBS B0034. When ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

GFP expression assay shows activity of K314100 as compaired to other protein expression cassettes.

Figure 1. GFP fluorescence was measured (ex. 485, em. 525) and normalized to cell density, read as the optical density at 600nm.
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