Generator

Part:BBa_K346087

Designed by: miao jing   Group: iGEM10_Peking   (2010-10-15)
Revision as of 01:45, 23 October 2010 by Starshine (Talk | contribs)

RBS(BBa_B0034)+MerP

This part is constructed for the expression of merP, which can bind Hg(II) in the periplasm and deliver it to merT for the transmembrane transportation. Structural studies have indicated that merP functions as a monomer and binds a single Hg (II) ion via its two cysteine residues at positions 14 and 17. It then transfer the Hg(II) to the merT, which receive it by a cysteine pair in the periplasm side.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None