Device

Part:BBa_K427009

Designed by: Jan Marte Contreras Ortiz   Group: iGEM10_Tec-Monterrey   (2010-10-22)
Revision as of 21:55, 22 October 2010 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Intelligent arabinose biosensor with differential response

This part was the final construct of the Tec-Monterrey iGEM Team in 2010. It senses the concentration of arabinose through the PBad family of promoters. Then amplifies it through sensitivity tuners, the activator proteins of the tuners are jammed by reverse arabinose promoters. The sensitivity tuners activate the production of the three fluorescent proteins. This device was created as a proof of concept for the combination of the jammers and sensitivity tuners, rather than using the whole part, the construct design is what should be regarded.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 228
    Illegal NheI site found at 655
    Illegal NheI site found at 1020
    Illegal NheI site found at 1306
    Illegal NheI site found at 1671
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 168
    Illegal BamHI site found at 715
    Illegal BamHI site found at 960
    Illegal BamHI site found at 1366
    Illegal BamHI site found at 1611
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2975


[edit]
Categories
Parameters
None