Reporter

Part:BBa_K316004

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-20)
Revision as of 16:37, 22 October 2010 by Ks907 (Talk | contribs)

Functional XylE under J23101 promoter, with double terminator

Constitutive E.coli promoter BBa_J23101 combined with BBa_K316003for easy characterisation of gene activity (catechol breakdown into 2,4 hydroxyaminobutane yellow product) under a standard E.coli promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 380
    Illegal NgoMIV site found at 552
    Illegal AgeI site found at 903
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aFunctional XylE under J23101 promoter, with double terminator