Measurement

Part:BBa_K337046

Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R   Group: iGEM10_Heidelberg   (2010-10-22)
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pSMB_miTuner Plasmid HD8 (BGH(rc)/shRNA10(rc)/RSV(rc)/CMV_TetO2/Luc2_sv40/RSV/Kozag_hRluc_BGH )

miTuner plasmid used for microRNA binding site characterization and expression tuning.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1659
    Illegal BamHI site found at 1
    Illegal BamHI site found at 3734
    Illegal XhoI site found at 3476
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1860
    Illegal NgoMIV site found at 3204
    Illegal NgoMIV site found at 3225
    Illegal NgoMIV site found at 3497
    Illegal AgeI site found at 2928
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4923
    Illegal SapI.rc site found at 3110


[edit]
Categories
Parameters
None