Other
Part:BBa_K337033
Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R Group: iGEM10_Heidelberg (2010-10-22)
Luc2_sv40/CMV(fw)
SV40 promoter driving a firefly luciferase reporter gene. Those two elements all rc, CMV promoter near SV40 drives in the opposite direction (fw).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1944
Illegal XhoI site found at 1686 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 70
Illegal NgoMIV site found at 1414
Illegal NgoMIV site found at 1435
Illegal NgoMIV site found at 1707
Illegal AgeI site found at 1138 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1320
[edit]
Categories
Parameters
None |