Coding

Part:BBa_K360022

Designed by: Claudia Ivonne Hernandez Armenta   Group: iGEM10_UNAM-Genomics_Mexico   (2010-10-21)
Revision as of 00:20, 22 October 2010 by Kurupaclau (Talk | contribs)

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Lumazine

Lumazine is a Protein that affect the wavelength of the emitted light from the LuxABCDE operon, it has been isolated from Photobacterium phosphoreum. The lumazine proteins shift the color of the light to shorter wavelengths than 490 nm, around 475 nm.

Lumazine is already at the registry page, submitted by Edinburgh team 2009.See the Part entry BBa_K216007.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 345
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

We decided to synthesize this part.

References

O'Kane, D.J., Woodward, B., Lee, J., and Prasher, D.C. 1990. Borrowed proteins in bacterial bioluminescence. Proc. Natl. Acad. Sci. USA 88, 1100-1104.

Lee, J., Wang, Y., and Gibson, B.G. 1991. Electronic excitation transfer in the complex of lumazine protein with bacterial bioluminescence intermediates. Biochemistry 30, 6825-6835.

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Parameters
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