Coding
Tse2

Part:BBa_K314200:Experience

Designed by: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger   Group: iGEM10_Washington   (2010-10-18)
Revision as of 23:03, 21 October 2010 by Swanson (Talk | contribs) (→‎Applications of BBa_K314200)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K314200

Tse2 is a potent bacterial toxin and thus has many applications, with or without its immunity protein, Tsi2. One potential application is use as a biotechnology agent in the way that the CcdB, the Death Protein, is used.

Tse2 could also be combined with the T6SS to result in a system that targets this toxin into the cytoplasm of target Gram-negative bacteria. This is useful to create Gram-negative bacteria that have been directed to target other Gram-negative bacteria for killing in response to a user-defined signal.

If Tse2 is combined with a functioning Type 6 Secretion System and the antitoxin Tsi2 it results in a killing system that can be targeted to Gram-negative bacteria. This could be used in a probiotic application. This was the goal behind the Gram-negative portion of the [http://2010.igem.org/Team:Washington 2010 UW iGEM project].

Diagram of the Tse2/Tsi2/ Type VI Secretion System as it would be used in a probiotic application

User Reviews

UNIQ7f6ff2461984af46-partinfo-00000000-QINU UNIQ7f6ff2461984af46-partinfo-00000001-QINU