Primer

Part:BBa_K300978

Designed by: Manuel Lupotto, Matteo Meroso, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-20)
Revision as of 16:46, 21 October 2010 by Matteo (Talk | contribs)

Phi80 locus reverse primer

This primer (in house known as P4) anneals in the genomic region flanking the attB(Phi80) site and can be used together with BBa_K300975 to amplify the entire Phi80 locus or verify the integration into the E. coli genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None