Coding
ladA

Part:BBa_K398000

Designed by: Mathias Voges   Group: iGEM10_TU_Delft   (2010-06-12)
Revision as of 09:41, 21 October 2010 by Mvoges (Talk | contribs)

Long-Chain Alkane Monooxygenase

A flavoprotein alkane monooxygenase native to Geobacillus thermodinitrificans NG-80-2. It has been found to specifically oxidize the terminal regions of alkanes ranging from C15 up to at least C36. The product is the corresponding primary alkanol. LadA forms a catalytic complex with flavin mononucleotide (FMN) which utilizes dioxygen to insert an oxygen atom into the substrate.

The general catalytic function involves three chemical processes:

  • Reduction of the cofactor flavin mononucleotide (FMN to FMNH2) by NAD(P)H
  • Reaction of FMNH2 with O2
  • Binding, orienting, and activating the substrate for its oxygenation

LadA's ability to preferentially capture long-chain alkanes for oxidation sets it apart from other flavoprotein monooxygenases[1].

File:LadA-dimer.jpg
1% agarose of colony PCR. Gel run at 100V for 50 minutes. Of all samples 5 µL was loaded with 1 µL loadingbuffer. 5 µL was loaded of marker


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 908


[edit]
Categories
//function/degradation
Parameters
familyFlavoprotein alkane monooxygenase