Device

Part:BBa_K341456:Design

Designed by: Teng Li   Group: iGEM10_Tsinghua   (2010-10-21)
Revision as of 07:25, 21 October 2010 by Registry (Talk | contribs)

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Kanamycin resistance(with Promoter) + I-sceI site+ Recombination site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 893
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 169
    Illegal SapI site found at 379
    Illegal SapI site found at 1225


Design Notes

This part involves DraIII cutting site at two ends of the unit, this cutting site is compatible with BBF RFC 61


Source

Kanamycin resistance gene is form plasmid "pKD13"

References