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Part:BBa_K314203:Design

Designed by: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger   Group: iGEM10_Washington   (2010-10-19)
Revision as of 04:07, 21 October 2010 by Swanson (Talk | contribs) (→‎Design Notes)

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HSL inducible toxin antitoxin, Tse2 + Tsi2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1283
    Illegal NgoMIV site found at 1340
    Illegal NgoMIV site found at 1465
    Illegal NgoMIV site found at 1795
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal SapI.rc site found at 1790


Design Notes

Note: This part does not contain the standard biobricks scar between Lux pR and B0034 and between B0034 and Tse2/Tsi2. Instead of the standard biobrick scar at these position, this part contains the Bglbrick scar GGATCT.

This construct was originally tested in pSB3K3 with Bglbrick prefixes and suffixes. We cloned it into pSB1C3 as per the Registry's request.

Source

Pseudomonas aeruginosa

References

Cell Host Microbe. 2010 Jan 21;7(1):25-37