Coding
Tse2
Part:BBa_K314200
Designed by: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger Group: iGEM10_Washington (2010-10-18)
Toxin Tse2
A toxic protein originating from Pseudomonas aeruginosa that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly. It is a substrate of the Pseudomonas aeruginosa type 6 secretion system. Tse2 has an immunity protein, Tsi2, that, when expressed in conjunction with Tse2 prevents cell death.
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria [http://www.ncbi.nlm.nih.gov/pubmed/20114026]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 196
Illegal NgoMIV site found at 253
Illegal NgoMIV site found at 378 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli/nissle
//collections/probiotics
//collections/probiotics/biocontainment
//collections/probiotics
//collections/probiotics/biocontainment
None |