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Part:BBa_K358006:Design

Designed by: Ken Kajita   Group: iGEM10_Kyoto   (2010-09-19)
Revision as of 22:45, 19 October 2010 by V08ken (Talk | contribs) (→‎Design Notes)

lambda lysis cassette with terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed PCR to get the lysis cassette[SRRz] and inserted before double terminator[BBa_B0015].

As the template, we used λ -Hind Ⅲ digest[http://catalog.takara-bio.co.jp/PDFFiles/3403_DS_j.pdf].

Forward primer:


Reverse primer:

Source

BBa_K358004 and BBa_B0015

References