Composite
Part:BBa_K358019:Design
Designed by: Ken Kajita Group: iGEM10_Kyoto (2010-10-19)
Lysis cassette regulated by lacP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Because of the lysis cassette, lactose promoter must be repressed when transform it. So, we used KRX, not Top 10. This part is toxic to E.coli.
Source
biobrick, lambda phage DNA