Regulatory

Part:BBa_K354000:Design

Designed by: Gregory Meyer   Group: iGEM10_WITS-South_Africa   (2010-10-19)
Revision as of 15:34, 19 October 2010 by Registry (Talk | contribs)

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Lac/Ara-1 IPTG Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The affiliation between AraC and its binding sequence had to be ensured so as to prevent basal expression and false triggering of the promoter.


Source

The promoter was synthesised via primer extension.

References