Regulatory
Part:BBa_K354000:Design
Designed by: Gregory Meyer Group: iGEM10_WITS-South_Africa (2010-10-19)
Lac/Ara-1 IPTG Inducible Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The affiliation between AraC and its binding sequence had to be ensured so as to prevent basal expression and false triggering of the promoter.
Source
The promoter was synthesised via primer extension.