RNA
Part:BBa_K411001:Design
Designed by: Po-Hsiang Liao Group: iGEM10_NYMU-Taipei (2010-10-15)
Theophylline-inducible Riboswitch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We want to choose a kind of riboswitch which has the following characteristics: The inducer cannot metabolize in E. coli. It does not exist in naturally occurring E. coli It does not have EcoRI,X,S, or PstI cutting site
Source
Synthesized directly from two primers:
- forward primer : 5' - gaattcgcggccgcttctagag ggtgataccagcatcgtcttgatgcccttggcag - 3'
- reverse primer : 5' - ctgcagcggccgctactagta cttgttgtcttgcagcggggtgctgccaagggcatcaagac - 3'