Regulatory

Part:BBa_K319005:Design

Designed by: Afnan Azizi   Group: iGEM10_uOttawa   (2010-10-16)
Revision as of 21:05, 16 October 2010 by Registry (Talk | contribs)

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yeast mid-length ADH1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 93


Design Notes

There were three options for the length of the promoter - short, middle, and long. The middle length was chosen.


Source

The part was extracted from S. cerevisiae, YPH500 strain, using colony PCR protocols. It consists of 720 bp upstream of ADH1 gene according to SGD sequence.

References