Project
rhlAB

Part:BBa_K424018:Design

Designed by: Team Panama iGEM 2010   Group: iGEM10_Panama   (2010-10-11)
Revision as of 00:19, 15 October 2010 by Ernestopro (Talk | contribs) (→‎Source)

Rhamnosiltransferase BioBrick (Rh1AB_BB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 169
    Illegal BamHI site found at 729
    Illegal XhoI site found at 905
    Illegal XhoI site found at 2191
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1084
    Illegal NgoMIV site found at 1805
    Illegal NgoMIV site found at 1918
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 394
    Illegal BsaI site found at 1434
    Illegal BsaI.rc site found at 578


Design Notes

The RhlAB gene complex have shown illegal restriction sites by the restriccition enzymes of the assembly standard protocol 10. We face this problem by applying the mutagenesis process that consist in doing silent mutation that change the nucleotides of our gene that are cutted by the restriction enzymes to made able the construction of our BioBrick.


Source

Pseudomonas aeruginosa from INDICASAT lab. and the genomic sequence of RhlAB was reviwed in genBank http://www.ncbi.nlm.nih.gov/nuccore/L28170.1

References

Qinhong Wang, Xiangdong Fang, Baojun Bai, Xiaolin Liang, Patrick J. Shuler, William A. Goddard III, Yongchun Tang. 2007. Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced Oil Recovery. Biotechnology and Bioengineering Volume 98, Issue 4, pages 842–853.