Generator
B-carotene

Part:BBa_K343006

Designed by: Christian Kurtzhals   Group: iGEM10_SDU-Denmark   (2010-10-14)
Revision as of 15:04, 14 October 2010 by Registry (Talk | contribs)

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Expresses B-carotene monooxygenase on a constitutive promotor

This generator expresses a beta-carotene 15,15'-monooxygenase,on a constitutive promotor, that catalyses retinal production from beta-carotene via the following reaction:

beta-carotene + O2 = 2 retinal


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 765
    Illegal BamHI site found at 500
    Illegal BamHI site found at 1757
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1361
    Illegal BsaI site found at 1809


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
//classic/generator/prct
//classic/generator/uncategorized
//function/biosynthesis/isoprenoid
Parameters
functionRetinal Biosynthesis
input_sBeta-Carotene
outputAll-trans retinal