Coding

Part:BBa_K313002

Designed by: Kohaku So, Takashi Hiroi, Megumi Iseki, Mami Nakatake, Ryosuke Kamei, Ryo Kariyazono   Group: iGEM10_UT-Tokyo   (2010-10-14)
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flpe DNA recombinase

Flpe is a site-specific DNA recombinase that that recognizes FRT sequence. Flpe was derived from yeaset site-specific DNA recombinase Flp. (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))

It's internal EcoRI and SpeI site has been removed using site-directed mutagenesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 504
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/flp
Parameters
None