Device

Part:BBa_K329008:Design

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-12)
Revision as of 19:54, 12 October 2010 by Rpantier (Talk | contribs) (→‎Design Notes)

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Double terminator (B0015) - attC - mRFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 205
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 205
    Illegal NotI site found at 1081
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 205
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 205
    Illegal AgeI site found at 784
    Illegal AgeI site found at 896
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS-mRFP is situated between the SpeI and PstI restriction sites, the excision of this gene permit to restore a normal biobrick suffix. We used BioBrick Standard Assembly.

Source

This construct was originally integrated in a pSUlib vector (low copy plasmid, Kanamycin resistant) according to the biobrick standard.

References