Reporter
luc

Part:BBa_K389004

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-08-10)
Revision as of 15:46, 12 October 2010 by Jaretz (Talk | contribs)

Luciferase from pGL4.10[luc2]

A firefly (Photinus pyralis) luciferase from Promega's pGL4.10luc2 vector. Luciferase is a sensitive reporter gene.

Usage and Biology

The activity of a luciferase is measured in a luminometer - so only use this BioBrick if you have one in your lab.

Firefly luciferase oxidizes its substrate, firefly luciferin, in presence of ATP and oxygen, emitting light (Conti et al., 1996). Because the firefly luciferin does not pass the cell membrane of E. coli under "normal" cultivation conditions the cells have to be lysated before the luciferase activity measurement.


Structure of Photinus pyralis firefly luciferase. Image taken from: http://en.wikipedia.org/wiki/Luciferase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 1408
    Illegal NgoMIV site found at 1429
    Illegal AgeI site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1314


[edit]
Categories
//cds/enzyme
//chassis/prokaryote/ecoli
//classic/reporter
//function/reporter/light
Parameters
None