Coding

Part:BBa_K343000

Designed by: Lars Christian Lund   Group: iGEM10_SDU-Denmark   (2010-07-02)
Revision as of 13:26, 12 October 2010 by Mbarnkob (Talk | contribs)

Mutated flagella master regulator (FlhDC mut).

The master regulator operon for flagellum synthesis. Produces transcriptional factors for Class II flagellar regulon genes. To make the part conform to the iGEM assembly standards we introduced a silent mutation, in an unintended restriction site, at BP 822 from t to c, so that Pst1 would not cleave the DNA there.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


Safety

[http://iai.asm.org/cgi/reprint/75/7/3315 Role of Motility and the flhDC Operon in Escherichia coli MG1655 Colonization of the Mouse Intestine]

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93725/ The Yersinia enterocolitica Motility Master Regulatory Operon, flhDC, Is Required for Flagellin Production, Swimming Motility, and Swarming Motility]

[http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0008029 Repression of Invasion Genes and Decreased Invasion in a High-Level Fluoroquinolone-Resistant Salmonella Typhimurium Mutant]


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Categories
Parameters
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