Translational_Unit
402

Part:BBa_K398402:Design

Designed by: Hugo Federico Cueto Rojas   Group: iGEM10_TU_Delft   (2010-10-12)
Revision as of 10:00, 12 October 2010 by Hugo 87 (Talk | contribs) (→‎Design Notes)

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Prefoldin-alpha generator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Enhanced sequence for expression on E. coli K12, restriction sites for the enzymes XbaI, PstI, EcRI and SpeI are removed from the CDS. An extra XbaI site will be found between RBS and Promoter so you can increase the expression level of the protein by changing the promoter.

Source

The NCBI genomic sequence was in silico enhanced for iGEM purposes

References