Coding

Part:BBa_K310007:Design

Designed by: Erin Borchardt   Group: iGEM10_UIUC-Illinois   (2010-09-24)
Revision as of 00:21, 12 October 2010 by Eborcha2 (Talk | contribs) (→‎Design Notes)

ArsR repressor of the Ars Operon in E. coli K-12 MG1655


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 138


Design Notes

Site directed mutagenesis was performed in the coding region of ArsR at nucleotide 139 due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT.

Source

E. coli K-12 MG1655 genome

References