Composite

Part:BBa_K389412:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-11)
Revision as of 14:35, 11 October 2010 by Registry (Talk | contribs)

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vir promoter + sensitivity tuner + luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2428
    Illegal NgoMIV site found at 3772
    Illegal NgoMIV site found at 3793
    Illegal AgeI site found at 1951
    Illegal AgeI site found at 2063
    Illegal AgeI site found at 2265
    Illegal AgeI site found at 3496
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3678


Design Notes

sensitivity tuner to enhance the luciferase signal


Source

  • luciferase from pGL4.10luc2 (Promega)
  • sensitivity tuner part, terminators and constitutive promoter from the parts.igem
  • virG was synthesized by Mr. Gene
  • vir promoter from Agrobacterium tumefaciens C58, TI-plasmid

References