Composite
Part:BBa_K389412:Design
Designed by: Jonas Aretz Group: iGEM10_Bielefeld-Germany (2010-10-11)
vir promoter + sensitivity tuner + luciferase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2428
Illegal NgoMIV site found at 3772
Illegal NgoMIV site found at 3793
Illegal AgeI site found at 1951
Illegal AgeI site found at 2063
Illegal AgeI site found at 2265
Illegal AgeI site found at 3496 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3678
Design Notes
sensitivity tuner to enhance the luciferase signal
Source
- luciferase from pGL4.10luc2 (Promega)
- sensitivity tuner part, terminators and constitutive promoter from the parts.igem
- virG was synthesized by Mr. Gene
- vir promoter from Agrobacterium tumefaciens C58, TI-plasmid