Composite

Part:BBa_K389411

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-11)
Revision as of 14:33, 11 October 2010 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


vir promoter + sensitivity tuner + luciferase

This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2428
    Illegal NgoMIV site found at 3772
    Illegal NgoMIV site found at 3793
    Illegal AgeI site found at 1951
    Illegal AgeI site found at 2063
    Illegal AgeI site found at 2265
    Illegal AgeI site found at 3496
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3678


[edit]
Categories
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//function/reporter/light
//regulation/positive
Parameters
None