Reporter

Part:BBa_K330002

Designed by: LI Zhuo, JIANG Hanlun and LU Wei   Group: iGEM10_HKUST   (2010-10-10)
Revision as of 13:54, 10 October 2010 by Registry (Talk | contribs)

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gusA reporter gene

The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme from Escherichia coli.

Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacteria. There are three often used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays varies (5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay, 4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl β-D-glucuronide for spectrophotometric assay).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter
Parameters
originE.coli
tagNone