Composite

Part:BBa_K318502

Designed by: Sarah R. Sandock   Group: iGEM10_Wisconsin-Madison   (2010-09-27)
Revision as of 23:03, 8 October 2010 by Sandock (Talk | contribs)

lacI pL + RBS + RcsB + RBS + RcsA + TT

Our goals is to have each cell produce enough colonic acid to protect itself from the harsh acidic conditions of the stomach. We placed RcsA, RcsB, and a combination of the two under a IPTG inducible promoter to test both quantity of colonic acid produced and cell survivability. You can see our data by downloading the associated .zip file for any of these three devices:BBa_K318500(RcsA), BBa_K318501(RcsB), BBa_K318502(RcsA+RcsB)

Usage and Biology

RcsA and RcsB are transcription factors that are positive regulator of capsular polysaccharide synthesis. The two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis. RcsB is also know to form a homodimmer and positivly regulate cell division. RcsA and RcsB belong to the multicomponent RcsF/RcsC

See more on http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA 

& http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10821 Ecocyc-RcsB

Characterization

Zip File Download

(Testing Data Here)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None