Generator

Part:BBa_K395706

Designed by: Yumiko Kinoshita   Group: iGEM10_Tokyo_Tech   (2010-10-08)
Revision as of 12:33, 8 October 2010 by Registry (Talk | contribs)

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rbs+crtZW under pBad promoter for double plasmids

This part contains crtZW, which works as the astaxanthin biosynthesis pathway when add beta-carotene as a substrate.

It is necessary to add either beta-carotene or the synthesis operon.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2405
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2488
    Illegal SapI site found at 961
    Illegal SapI site found at 2315
    Illegal SapI site found at 2327


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Categories
Parameters
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