Coding

Part:BBa_K322246:Design

Designed by: Meng Lu and William Rostain   Group: iGEM10_Edinburgh   (2010-09-24)
Revision as of 19:22, 7 October 2010 by WillR (Talk | contribs) (→‎Design Notes)

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Red emitting firefly luciferase mutant (S284T)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


Design Notes

This luciferase seems to be temperature sensitive, and growing the host at 30°C rather than 37°C considerably improves brightness.

Source

Photinus pyralis

References

Shapiro, E., Lu, C. & Baneyx, F. (2005). A set of multicolored Photinus pyralis luciferase mutants for in vivo bioluminescence applications. Protein Engineering, Design and Selection 18, 581-587.