Coding

Part:BBa_K322237:Design

Designed by: Chris French and William Galdric Rostain   Group: iGEM10_Edinburgh   (2010-09-26)
Revision as of 19:15, 7 October 2010 by WillR (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Firefly luciferase from Photinus pyralis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


Design Notes

The suffix seems to be missing a base between the stop codon and the SpeI site. It seem this was missing in the original clone.

Source

The origional sequence is from Photinus pyralis, a the gene here is derived from BioBrick BBa-I712019.

References