Coding

Part:BBa_K322211:Design

Designed by: William Rostain and Meng Lu   Group: iGEM10_Edinburgh   (2010-09-24)
Revision as of 18:49, 7 October 2010 by WillR (Talk | contribs) (→‎References)

Red emitting firefly luciferase mutant (356K)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


Design Notes

Sequence date: the mutation was initially introduced into our construct K216015 and the sequence across the mutation was obtained on 12 Aug 2010 (clone 4). The coding sequence was then amplified by PCR and cloned in pSB1C3 by Will and Lu.


Source

Photinus pyralis, from BBa_I712019

References

Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136. Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007).