Composite

Part:BBa_K389012:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-09-11)
Revision as of 12:39, 7 October 2010 by Jaretz (Talk | contribs) (→‎Design Notes)

VirA reporter system luc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1195
    Illegal NgoMIV site found at 2539
    Illegal NgoMIV site found at 2560
    Illegal AgeI site found at 2263
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2445


Design Notes

  • medium strong constitutive promoter
  • double terminator (forward) to keep basal expression of kanamycin resistance low
  • luciferase gene to show the activity of the vir promoter

Source

parts.igem, synthetic gene by Mr. Gene, pGL4.10luc2 by Promega, TI-plasmid from Agrobacterium tumefaciens C58

References