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Sample It's complicated

Experience: Works

Composite

Part:BBa_K389011:Design

Designed by: Jonas Aretz Group: iGEM10_Bielefeld-Germany (2010-08-10)

Revision as of 12:34, 7 October 2010 by Jaretz (Talk | contribs) (→‎Source)

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VirA screening device

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

medium strong constitutive promoter,
double terminator (forward) to keep basal expression of kanamycin resistance low
- double terminator BBa_B0017 instead of BBa_B0015 because of problems mentioned with BBa_B0012
kanamycin resistance for screening with plates

Source

virG gene synthesized by Mr. Gene (BBa_K389002)
vir promoter from A. tumefaciens C58 (BBa_K389003)
kanR gene made from BioBrick BBa_P1003
constitutive promoter and double terminator from parts.igem (BBa_J23110, BBa_B0017)

References