Composite

Part:BBa_K389011:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-08-10)
Revision as of 12:33, 7 October 2010 by Jaretz (Talk | contribs) (Source)

VirA screening device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • medium strong constitutive promoter,
  • double terminator (forward) to keep basal expression of kanamycin resistance low
  • kanamycin resistance for screening with plates

Source

  • virG gene synthesized by Mr. Gene (BBa_K389002)
  • vir promoter from A. tumefaciens C58 (partinfo>K389003</partinfo>)
  • constitutive promoter and double terminator from parts.igem (BBa_J23110, BBa_B0017)

References