Generator
virA

Part:BBa_K389010:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-08-10)
Revision as of 12:01, 7 October 2010 by Jaretz (Talk | contribs) (→‎Design Notes)

VirA receptor (expressed)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768


Design Notes

The virA gene is under the control a medium strong constitutive promoter so there is enough VirA receptor expressed but not too much so the cell suffers from the expression (VirA is a membrane protein)

Source

A. tumefaciens C58, parts.igem

References