Coding
eIF4A

Part:BBa_K411100:Design

Designed by: Geng Ming Su   Group: iGEM10_NYMU-Taipei   (2010-10-06)
Revision as of 07:13, 7 October 2010 by Blackrabbit (Talk | contribs) (→‎Design Notes)

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Eukaryote Initiation Factor 4A (eIF4A) from Mus Musculus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 240
    Illegal BglII site found at 536
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 972
    Illegal BsaI.rc site found at 638


Design Notes

Mutated two PstI cutting sites at positions 223 and 391 from ctgcag to ctccag.

Source

[http://www.ncbi.nlm.nih.gov/nuccore/NM_144958 eIF4A] from Mus Musculus

References