Device

Part:BBa_K302018

Designed by: Steven Woodhouse, Alan Koh, Rachel May Boyd, Philip Hall   Group: iGEM10_Newcastle   (2010-08-18)
Revision as of 15:13, 28 September 2010 by Swoodhouse (Talk | contribs)

Subtilin immunity

Subtilin is a lantibiotic peptide produced naturally by Bacillus subtilis ATCC 6633 but not by Bacillus subtilis 168.

This part encodes two separate subtilin immunity systems — a membrane bound protein that binds subtilin and prevents it from penetrating the cell membrane to form pores, and a transporter which removes subtilin from the membrane), which together provide a high degree of subtilin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 837
    Illegal BglII site found at 1585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1132
    Illegal SapI site found at 1412
    Illegal SapI.rc site found at 2135


[edit]
Categories
Parameters
None