Device
"Lock"

Part:BBa_K318030:Design

Designed by: Justin Vrana, Nate Pantalone   Group: iGEM10_Wisconsin-Madison   (2010-07-13)
Revision as of 20:22, 27 September 2010 by N8pants (Talk | contribs) (→‎Design Notes)

lox66 + rpCons + hixC + lox71 + SapI + T + T + Hin enhancer + HindIII + hixC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 43
    Illegal NheI site found at 66
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 162


Design Notes

Note:

  • The constitutive promoter is oriented to express whatever gene is downstream of the final hixC site after recombination.
  • The SapI site located within the part yields a cut site as shown 5'(A\ATCT)3' on top and 3'(TTAG\A)5' on bottom.
  • The HindIII site located within the part actually allows for "lock breaking" because inserting a promoter into the part through standard cloning protocols bypasses the "lock" effect. The site was added to more easily recognize different recombinants through restriction digests.

Source

From Synthesis

References