Generator

Part:BBa_K318000

Designed by: Nathaniel Pantalone, Justin Vrana   Group: iGEM10_Wisconsin-Madison   (2010-08-27)
Revision as of 20:09, 27 September 2010 by N8pants (Talk | contribs)

pBad + Cre + pLac + Hin + pCons + lacI

This composite part is an inducible recombinase generator. It can produce the Cre recombinase if induced with arabinose, and it can produce the Hin recombinase if induced with IPTG or an allo-lactose equivalent. It was refered to as the "Key Cassette" or "Key Construct" by the Wisconsin-Madison 2010 iGEM team.

It can work in conjunction with the Lock Cassette (after junk DNA adjusts the lock to the optimal length). The correct "combination" is IPTG induction and then arabinose induction, expressing the Hin and Cre recombinases respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1252
    Illegal NheI site found at 3141
    Illegal NheI site found at 3164
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4342
    Illegal BamHI site found at 1191
    Illegal BamHI site found at 1642
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1026
    Illegal AgeI site found at 1328
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1008


[edit]
Categories
Parameters
None