Coding

Part:BBa_K385003:Design

Designed by: Krystal Annand, Joseph Hoare, Justyna Kucia, Stephen Lam   Group: iGEM10_Aberdeen_Scotland   (2010-09-22)
Revision as of 11:38, 27 September 2010 by I.stansfield (Talk | contribs) (→‎Design Notes)

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Phage lambda N-peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 29


Design Notes

The part was engineered with an AUG, but no stop codon, to allow the part to be used as a translational fusion with another downstream open reading frame. A glycine rich spacer peptide was inserted at the 3' end of the sequence, to allow the N-peptide to be separated from any downstream ORF by a flexible linker. (Linker sequence GGT GAC GGT GCT GGT TTA ATT AAC)

Source

Phage lambda genome

References