Coding

Part:BBa_K385002:Design

Designed by: Krystal Annand, Joseph Hoare, Justyna Kucia, Stephen Lam   Group: iGEM10_Aberdeen_Scotland   (2010-09-22)
Revision as of 11:38, 27 September 2010 by I.stansfield (Talk | contribs) (→‎Design Notes)

Phage MS2 coat protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 331


Design Notes

We omitted the stop codon so this part could be used in a protein fusion construct, with the MS2 protein forming the N-terminal domain. A glycine rich spacer peptide was inserted at the 3' end of the sequence, to allow the N-peptide to be separated from any downstream ORF by a flexible liker. (Linker sequence GGT GAC GGT GCT GGT TTA ATT AAC)

Source

Phage MS2 genome (see NCBI sequence http://www.ncbi.nlm.nih.gov/nuccore/V00642.1)

References