Coding

Part:BBa_K331020:Design

Designed by: Adam Smith   Group: iGEM10_Lethbridge   (2010-09-26)
Revision as of 23:20, 26 September 2010 by Adam.smith4 (Talk | contribs) (→‎Source)

Catechol 2,3 Dioxygenase with Fusion Prefix


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 311
    Illegal NgoMIV site found at 483
    Illegal AgeI site found at 834
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was important that we removed the endogenous P. putida ribosomal binding site, as well as the promoter from the sequence in order to allow for the creation of a fusion protein.


Source

This part is the PCR product of BBa_K118021 using the following primers:
Forward: 5'-GAA TTC GCG GCC GCT TCT AGA ATG AAC AAA GGT GTA ATG CGA CCG-3' (xylE Prefix Fusion)
Reverse: 5'-CTG CAG CGG CCG CTA CAT GTA GGT CAG CAC GGT CAT GAA TCG-3' (xylE Suffix Standard)

References