Coding

Part:BBa_K322246

Designed by: Meng Lu and William Rostain   Group: iGEM10_Edinburgh   (2010-09-24)
Revision as of 11:51, 26 September 2010 by Shenalu (Talk | contribs)

Red emitting firefly luciferase mutant (S284T)

This is the same as the other BioBrick BBa-K322211 but mutation S284T was introduced, which has been reported to result in a red-shifted output.

Features: S284T mutation 850 to 852

reference:Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136. Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


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Categories
Parameters
None