Translational_Unit
sacB

Part:BBa_K322921:Design

Designed by: Chris French and Maria Kowal   Group: iGEM10_Edinburgh   (2010-09-24)
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B. subtilis levansucrase. Lethal to E. coli in presence of sucrose.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note: MABEL was used to mutate an internal EcoRI site to GAGTTC (underlined). Features: coding sequence runs from 13 to 1434, RBS 1 to 3, EcoRI site mutated 211 to 216.



Source

Source: Bacillus subtilis 168 genomic DNA. Sequence (from SacBPlan11June10.docx)


References