Part:pSB1A10

Screening Plasmid v1.0

This plasmid can be used to do a rough characterization of the Input/Output curve for a PoPS-based device. Additionally, it can be used for screening of part or device libaries via FACS. More information can be [http://openwetware.org/index.php?title=Endy:Screening_plasmid_1.0 found here.]

Usage and Biology

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5242
  Illegal NheI site found at 4443
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 5248
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5242
  Illegal BglII site found at 89
  Illegal BamHI site found at 4382
  Illegal XhoI site found at 67
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 5242
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 5242
  Plasmid lacks a suffix.
  Illegal XbaI site found at 5257
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal AgeI site found at 674
  Illegal AgeI site found at 786
  Illegal AgeI site found at 4217
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2197
  Illegal BsaI.rc site found at 5118
  Illegal SapI site found at 4199
  

Functional Parameters

chassis	-NA-
copies	100-300
mcs	BioBrick
origin	pMB1
resistance	A

References

[2] Khlebnikov et al,Modulation of gene expression from the arabinose-inducible araBAD promoter. J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.

[3] Effect of gene location, mRNA secondary structures, and RNase sites on expression of two genes in an engineered operon. Biotechnol Bioeng. 2002 Dec 30;80(7):762-76.